Coding

Part:BBa_K5181005:Design

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-08-13)


FPPS_Santalum album


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

The sequence was codon optimized for Pseudomonas putida with Genscript codon optimizer. Amino Acid sequence: MGDRKTKFLEAYSVLKSELLRDPAFNFTDASRQWVDRMLDYNVPGGKLNRGLSVIDSYELLKEGKELTDDEIFLASALGWCIEWLQAYFLVLDDIMDGS HTRRGQPCWFRLPEVGLIAVNDGIMLRNHIPRILKKHFKNKPYYVELLDLFNEVEFQTTSGQMIDLITTLEGQKDLSKYSMPIHHRIVQYKTAYYSFYL PVACALLMSGENLDSHTEVEKILVEMGTYFQVQDDYLDCFGHPDVIGKIGTDIEDFKCSWLVVKALELSNEEQKKLLYENYGKADEASVAKVKALYKEL DLEGAFVEYENASYEKIISSIEVQPSKAVQAVLKSFLAKIYKRQKHHHHHH

The sequence has a 6X His-tage at its C-terminal end and can be purified by Ni-NTA columns.


Source

The part is obtained from NCBI(HQ343283.1) from Chavez et al. Biosynthesis of Sandalwood Oil: Santalum album CYP76F Cytochromes P450 Produce Santalols and Bergamotol (2013)

References